They were quartered at Fort Warren in Boston Harbor for most of their training, also for a short time at Camp Cameron in North Cambridge. They departed the Bay State on June 29, 1861
- Massachusetts Infantry Mustered in United States service for three years on 13 June, 1861, and arrived at Washington D. C. on 2 July, 1861
- Colonel G Clark, Jr.
- Lieutenant Colonel W Blaisdell
- Major G F Tileston
- Company A: Capt. M Moore
- Company B: Paul Revere Guard: Capt. J H Davis
- Two companies, under the command of Captain J H Davis, were assigned to escort the 1st United States Artillery, Light Company G, 2nd Section, to Sangster's Station, Virginia, at 4 PM on 16 July, 1861.
- Company C: Clark Light Guard: Capt. P D Tripp
- Company D: Capt. J W Butters
- Company E: Boston Light Guard: Capt. J R Bigelow
- Company F: Capt. L. Gordon
- Company G: Capt. W C Allen
- Company H: Sanford Light Guard: Capt. S Page
- Company I: Bunker Hill Volunteers: Capt. B F Wright
- Company K: Charlestown City Guard: B Stone, Jr.